Journal: Journal of Neuroinflammation

Article Title: Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy

doi: 10.1186/1742-2094-10-110

Figure Lengend Snippet: List and features of antibodies

Article Snippet: Anti-PMN , Cedarlane - CLAD51140 , 1:200.

Techniques:

Journal: iScience

Article Title: Differential Roles of the Calcium Ion Channel TRPV4 in Host Responses to Mycobacterium tuberculosis Early and Late in Infection.

doi: 10.1016/j.isci.2020.101206

Figure Lengend Snippet: Figure 7. Expression of Neutrophil Elastase in M. tuberculosis-Infected Mouse Lung (A–C) (A and B) WT and Trpv4/mice were infected with M. tuberculosis (100 CFU) via aerosol, and 4-mm cryosections of lung tissue samples were prepared after days 55 and 150 p.i. Tissue sections were stained by fluorescence immunohistochemistry assay for neutrophil elastase (green). Images were acquired from three different zones of infection (field) from each mouse; Scale bar=20 mm). Mean fluorescence intensity (MFI) of neutrophil elastase staining was quantified by using Fiji ImageJ software. (C) Human lung tissue sections of patients with TB were stained for TRPV4 showing positive signals in polymorphonuclear neutrophils in a blood vessel at the site of infection (Scale bar of R1, R2 = 20 mm).

Article Snippet: Primary antibodies directed against Trpv4 (#ACC034, Alomone lab), Rab5 (#GTX108605, GeneTex), neutrophil elastase (#51-862, proSci- ELAN)were used in this study.

Techniques: Expressing, Infection, Aerosol, Staining, Immunohistochemistry, Software

Journal:

Article Title: "Kinin danger signals proteolytically released by gingipain induce fimbriae-specific IFN-? and IL-17-producing T cells in mice infected intramucosally with Porphyromonas gingivalis "

doi: 10.4049/jimmunol.0900895

Figure Lengend Snippet: The graphs depicts the intensity of mucosal edema (buccal-lingual) in C57BL/6 WT (TLR2+/+) versus C57BL/6.TLR2−/− and C57BL/6 WT (B2R+/+) versus C57BL/6.B2R−/− mice that were inoculated 3 h earlier (mandibular anterior vestibule) with 30 µl of either P. gingivalis W83 wild-type (WT) strain, Rgps-deficient mutant (Δ Rgps) or the WT W83 bacteria pretreated with the Rgps inhibitor. Where indicated, the mice were pre-treated with HOE-140, prior to injection of the bacteria. A separate group of mice (C57BL/6 WT) were pretreated (18 h before bacteria injection) with 0.45 ml i.p. of a 1:10 dilution in PBS of rabbit antiserum to PMN or an equivalent volume of normal rabbit serum (control). The results (means ± SD) are representative of three independent experiments (n = 5 mice/group). Statistical differences between mean values were evaluated by ANOVA, and pair-wise comparisons were done by the Tukey test (*, p <0.01).

Article Snippet: Depletion of polymorphonuclear neutrophils (PMN) was sought by pretreating (− 18 h) the intrabuccally infected mice with 0.45 ml i.p. of a 1:10 dilution in PBS of rabbit antiserum to PMN (Accurate Chemical Corporation).

Techniques: Mutagenesis, Injection

Journal:

Article Title: "Kinin danger signals proteolytically released by gingipain induce fimbriae-specific IFN-? and IL-17-producing T cells in mice infected intramucosally with Porphyromonas gingivalis "

doi: 10.4049/jimmunol.0900895

Figure Lengend Snippet: Levels of IFN-γ (A) and IL-17 (B) secreted by submandibular LN Ag-specific T cells isolated from Balb/C mice orally infected (bottom of the mandibular anterior vestibule) either with wild-type P. gingivalis W83 or the Δ Rgps strain. Where indicated, mice were pre-treated with HOE-140, 1 h before the injection. A separate group of mice were depleted of circulating neutrophils 18 h before infection with 0.45 ml i.p. of a 1:10 dilution in PBS of rabbit antiserum to PMN or an equivalent volume of normal rabbit serum (control). PMN-depleted mice were either challenged with P. gingivalis W83 or Δ Rgps strain. In a second scheme, PMN-depleted mice were infected with P. gingivalis (wild-type and Δ Rgps strains) combined to human purified kininogen (HK), pre-treated or not with HOE-140, as indicated. After 10 d, the infected mice were boosted with Fim Ag (1 µg/mouse). Submandibular LN T cells were recovered and stimulated with Fim Ag (100 ng/ml) in vitro. Culture supernatants were collected after 72 h and IFN-γ and IL-17 were quantified by ELISA. Statistical differences between mean values were evaluated by ANOVA, and pair-wise comparisons were done by the Tukey test (*, p <0.01).

Article Snippet: Depletion of polymorphonuclear neutrophils (PMN) was sought by pretreating (− 18 h) the intrabuccally infected mice with 0.45 ml i.p. of a 1:10 dilution in PBS of rabbit antiserum to PMN (Accurate Chemical Corporation).

Techniques: Isolation, Infection, Injection, Purification, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Human Gene Therapy

Article Title: rVSV(M?51)-M3 Is an Effective and Safe Oncolytic Virus for Cancer Therapy

doi: 10.1089/hum.2007.163

Figure Lengend Snippet: Improved intratumoral rVSV replication and tumor necrosis with antibody-mediated depletion of neutrophils and NK cells in tumor-bearing rats. Buffalo rats harboring multifocal HCC lesions in the liver were intravenously injected with rabbit anti-rat polymorphonuclear leukocyte (PMN) antiserum (Wako Chemicals USA), polyclonal rabbit anti-asialo-GM1 (Wako Chemicals USA), or control rabbit IgG at a dose of 1 mg/200 μl/rat 1 day before viral infusion through the hepatic artery. A single injection of rVSV-LacZ or rVSV(MΔ51)-LacZ at 5.0 × 107 PFU/kg was performed the next day. The antibody injections were repeated 1 day after rVSV infusion (n = 3 for each group). The treated animals were killed 3 days after virus administration and hepatic lesions were collected for neutrophil and NK cell content determination by immunohistochemical staining and morphometric analyses, intratumoral viral titers by TCID50 assays, and tumor necrosis by histological staining followed by morphometric analyses. (A–C) After neutrophil depletion with rabbit anti-rat PMN antiserum; (D–F) after NK cell depletion with rabbit anti-asialo-GM1 antiserum. Data are shown as means and standard deviation, and statistical analyses were performed by Student t test.

Article Snippet: Neutrophil and NK cell depletion was accomplished by intravenous administration of rabbit anti-rat polymorphonuclear leukocyte (PMN) antiserum (Wako Chemicals USA, Richmond, VA) and polyclonal rabbit anti-asialo-GM1 (Wako Chemicals USA) 24 hr before as well as 24 hr after vector infusion.

Techniques: Injection, Immunohistochemical staining, Staining, Standard Deviation